Real-Time PCR

Real-time quantitative PCR, also known as qPCR, combines the PCR amplification and detection into a single step, thus eliminating the need to detect products using gel electrophoresis and enabling the PCR method to be truly quantitative. Real-time PCR instrument systems utilize fluorescent dyes to detect the accumulation of PCR products during the exponential phase of the reaction, which allows for fast and precise product quantification and objective data analysis.
Real-time PCR instruments are used to perform the PCR as well as the real-time detection and monitoring of the resulting fluorescent signal. The Applied Biosystems family of qPCR systems, combined with our gold standard qPCR master mixes and TaqMan® probe-based assays for gene expression and genetic variation, make qPCR more accessible than ever.


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