Ion Torrent Sequencing

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Ion Torrent technology uses a massively parallel array of proprietary semiconductor sensors to perform direct real-time measurement of the hydrogen ions produced during DNA replication. A high-density array of wells on the ion semiconductor chips provides millions of individual reactors while integrated fluidics allow reagents to flow over the sensor array. This unique combination of fluidics, micromachining, and semiconductor technology enables the direct translation of genetic information (DNA) to digital information (DNA sequence), rapidly generating large quantities of high quality data.

 

TECHNOLOGY

     
How is ti made? How does it work? How does it perform?
Scalability
How is it made?
Simplicity
How does it work?
Speed
How does it perform?

 

 

APPLICATIONS

The PGM™ system enables all possible sequencing applications. Perform multiplex amplicon sequencing in a record time. Moreover, the 200bp reads make sequencing long amplicons possible, resulting in more simple and affordable projects. Obtain optimal results for your microbial sequencing projects with extraordinary uniformity of coverage and long read lengths. The four to eight million reads produced by the 316 and 318 chips are ideal for applications such as transcriptome, small RNA sequencing, and ChIP-Seq. Paired-end sequencing, methylation and other applications are fully compatible with the PGM™ sequencer. The rapid hypothesis to result cycle empowers the researcher to push the limits of their creativity and obtain tangible results in record time.

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Example Applications

 

small genome sequencing

target capture

amplicon re-sequencing

miRNA

library assessment

RNA Seq

Small Genomes Sequencing Target Capture Targeted (Amplicon) Re-Sequencing RNA Seq Library Assessment RNA Seq
           

Whole Genome Whole Exome Validation

copy number

barcoded libraries

paired ends

Chip Seq

 

Whole Genome/ Whole Exome Validation Copy Number Barcoded Libraries Paired End Reads ChIP-Seq  

 

     
The IonTouch System  

ION TORRENT PRODUCTS

 
Ion Torrent technology uses a massively parallel array of proprietary semiconductor sensors to perform direct real-time measurement of the hydrogen ions produced during DNA replication. A high-density array of wells on the Ion Semiconductor Chips provides millions of individual reactors while integrated fluidics allow reagents to flow over the sensor array. This unique combination of fluidics, micromachining, and semiconductor technology enables the direct translation of genetic information (DNA) to digital information (DNA sequence), rapidly generating large quantities of high quality data.
 
Click a step below to view products.
 

Construct DNA LibraryPrepare TemplateSequence and Generate Base CallsAnalyze Data

 
 
 

 

 

 

 

 

NEW Automated Template

 
PrepWalk-away automation, the Ion OneTouch™ System* reduces hands-on time to as little as five minutes and total time to less than three hours—three times faster than with standard template prep.
 
The Ion Personal Genome Machine™ sequencer redefined the world of sequencing. Now, simpler template prep makes it more accessible for every lab. And there's more to come.
 
 
The Ion OneTouch™ System simplifies the workflow for the Ion Personal Genome Machine™ (PGM™) sequencer and enables reproducible template preparation in ~3 hours. The low-cost, benchtop system is comprised of two components: one combines amplification and Ion Sphere™ particle recovery into a single automated process, the other automates Ion’s proven magnetic enrichment procedure for isolating template-positive Ion Sphere™ particles. The Ion OneTouch™ System streamlines the overall Ion sequencing workflow, yields significant time savings, increases sequencing productivity, and accelerates scientific discovery.