Gene Expression Analysis

     
     
                                                 

When, where, and under what conditions are genes expressed? What triggers—or prevents—this expression? Scientists are discovering the surprisingly wide range of products of transcription and translation, and how these different expression products determine the growth and health of an organism.

 

Next-Generation Sequencing

The SOLiD™ Serial Analysis of Gene Expression (SAGE™) System provides a highly sensitive, hypothesis-neutral method for quantifying gene expression levels on a genome-wide scale

 

SOLiD™ SAGE™ System: For quantification of gene expression 

The SOLiD™ Serial Analysis of Gene Expression (SAGE™) System provides a highly sensitive, hypothesis-neutral method for quantifying gene expression levels on a genome-wide scale. This method, which determines mRNA expression levels by sequencing unique sequence tags isolated from the 3’ ends of mRNAs, has significant advantages over microarray technologies:

  • Detects known and novel mRNAs.
  • Is highly reproducible, with a dynamic range that is orders of magnitude greater than microarrays.
  • Detects expression of transcripts from < 1 copy per cell to over 100,000 copies per cell, corresponding to a dynamic range of > 105.

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Real-Time PCR

Applied Biosystems has developed two methods for gene expression analysis using real-time PCR instruments:

   

TaqMan® Probe-Based Gene Expression Analysis

  Gene Expression Assay Tubes
Single Tubes
Over 1,200,000 pre-designed TaqMan® Assays for quantitative gene expression in 19 species. Custom assays to study any gene or splice variant in any organism.
 
  384-Well Plates

96-Well Plates

Easy-to-use 96-well plates are compatible with our 7300, 7500, 7500 Fast, 7900HT, and StepOnePlus™ 
 
  96-Well Plates
384-Well Plates
384-well plates can be configured with any TaqMan® Gene Expression Assays, including custom assays designed to your target sequences.
 
  384-Well Microfluidic Cards
384-Well Microfluidic Cards
384-well cards are ideal for microarray validation or other large-scale studies, and are compatible with our ViiA™ 7 and 7900HT Real-Time PCR Systems.
 
  Greater than 384 Wells
Greater than 384 Wells
The OpenArray® Real-Time PCR System is our mid-density gene expression solution for studying hundreds of samples per day.
 
   

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SYBR® Green-Based Gene Expression Analysis

 

 
SYBR® Green-Based Detection Using Traditional Real-Time PCR Instruments
View our complete selection of real-time PCR reagent, kits and instruments.
 
 
SYBR® Green-Based Detection Using the OpenArray® Real-Time PCR System
Discover our mid-density gene expression solution.
 
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