High Content Screening & Cellular Imaging

   

 

Cellular imaging and high content screening technology is widely used in academia to measure cellular function including cell morphology and the function and behavior in cells. The high content imaging technology is also applied in the pharmaceutical and biotechnology industries to help advance the treatment of illnesses such as spinal cord injuries, neurological disorders, chronic inflammation, asthma, organ and tissue transplantation, and cancer.

A complete cellular imaging workflow consists of instruments and software working together to acquire and analyze images, then process, store, and manage the experimental data. Molecular Devices offers several complete integrated workflows for high content screening under its ImageXpress® and IsoCyte brands, supported by high content analysis software under its MetaXpress®, AcuityXpress™, and MDCStore™ brands. All of these screening solutions offer the advantages of intuitive acquisition, automated image analysis, turnkey cellular informatics, and the seamless integration of the hardware and software components, which eliminates manual data manipulation or transfer encountered in a less sophisticated imaging workflow.
Molecular Devices high content screening and high content imaging products are primarily used for target identification and validation, screening using functional genomics, quality control, assay development using fluorescent labels or GFP, and drug screening.
 
 
Please use the links below to learn more about how we can support the success of your high content screening efforts.
 

ImageXpress Micro Widefield High Content Screening System

The ultimate combination of speed and flexibility in a turnkey solution that streamlines research and drug discovery in a fully integrated high content screening environment.

ImageXpress® Micro System captures research-quality images with the widest range (1X to 100X) of objective lenses available in an HCS system, enabling you to work at the resolution appropriate for your biology, including small organism, cellular events, or intracellular events. The system's best-in-class 100 nm resolution stages precisely locate and identify sub-cellular features, ensuring excellent X, Y, and Z positioning to simplify tracing individual cells during multi-day time-lapse experiments or mapping neuronal outgrowth across multiple tiled images.

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ImageXpress Ultra Confocal High Content Screening System

A true point-scanning confocal microscope reliably automated as a turnkey solution for high-throughput imaging that streamlines the work flow required to conduct almost any cell-based screening project.

ImageXpress® Ultra HCS System is a fast, reliable, research-grade point scanning confocal solution that streamlines high-throughput imaging and hit selection in a fully integrated environment.  Utilizing the best X, Y, and Z positioning available, the system precisely locates and identifies sub-cellular features such as clathrin coated pits or tiles together high resolution images to quantify neuronal length.  Rapid positioning in combination with the laser autofocus option furnishes users with intuitive tools to quantify heterogeneous multi-parametric responses, improving the high content screening workflow.

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ImageXpress Velos Laser Scanning Cytometer

Experience the benefit of multiplexed, cell- and bead-based assays performed during high content screening in a high throughput screening environment, ensuring heterogeneous responses can be captured earlier in the research and discovery of new therapeutics.

multiplexed, homogeneous, bead and cell-based assays become easy to develop and run as robust primary screens. The cytometer can scan a plate in as little as 3 minutes, regardless of cell- or well-density while resolving objects at micron resolution in four colors simultaneously. These fast scan times and whole well analysis can gather data from >150,000 wells per 8-hour day, achieving the acquisition rates expected of high-throughput screening. The cytometer's speed comes from unique design elements including a large depth of focus and large field of view which eliminates the requirements to periodically focus or acquire multiple exposures to cover an entire field of view. A light scatter (label free) mode allows generation of data from non-fluorescent objects (e.g., stem cell colonies where addition of dyes is unacceptable).

Common assays include:

  • Apoptosis
  • Cell cycle
  • Cell proliferation
  • Cell Migration or Invasion
  • Cytotoxicity
  • Cell- or bead-based homogeneous assays
  • Hybridoma screening
  • Adherent cell cytometry
  • Single cell phospho-protein detection
  • Protein-protein interactions in living cells
  • Spot- and bead-based arrays in plates
  • Stem cell or primary cell surface marker expression
  • Toxicity in model organisms